Protein Expression and Purification Facility

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Full list of protocols
　　Analytical Ultracentrifugation
　　　Cell assembly
　　?quilibrium run
　　?elocity run
　　?ata analysis
　　Chromatography
　　?alibration of Superose 12 HR 10/30
　　?alibration of Superdex HR 10/30
　　?alibration of HiLoad 16/60 Superdex 75 prep grade
　　?alibration of HiLoad 26/60 Superdex 75 prep grade
　　?alibration of HiLoad 26/60 Superdex 200 prep grade
　　Cloning
　　?reparation of oligo solutions
　　?CR reactions
　　?igestion of insert DNA
　　?igestion and dephosphorylation of vector DNA
　　?igation of DNA fragments with sticky ends
　　?igation of DNA fragments with blunt ends
　　?reparation of competent E. coli cells
　　?ransformation of plasmid DNA to competent E. coli cells
　　Cloning by homologous recombination
　　　Creating a GATEWAY Entry Clone via the BP Reaction
　　　Creating a GATEWAY Expression Clone via the LR Reaction
　　　One tube protocol to create a GATEWAY Expression Clone
　　　2-Step GATEWAY PCR experiments
　　Extraction and Clarification
　　　Preparation of cell lysates from E. coli by sonication
　　　Preparation of cell lysates from E. coli using a French Press
　　　Preparation of cell lysates from E. coli by enzymatic lysis
　　　Preparation of cell lysates from yeast using a French press
　　　Preparation of cell lysates from yeast using glass beads vortexing
　　　Preparation of cell lysates from yeast by enzymatic digestion of the cell wall
　　　Solubilization and renaturation of proteins from inclusion bodies
　　Plasmid Stability
　　　Plasmid stability test
　　Protein labelling
　　　N15 labelling
　　　N15 and C13 labelling
　　　Seleno-methionine labelling
　　Protein toxicity
　　　\"lating\" method for culture inoculation
　　Purification
　　　His-tag protein purification using Ni-NTA magnetic beads

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