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Hepatocytes Methods and Protocols
Publisher: Humana Press
Author: Patrick Maurel
Language: English
ISBN: 1607616874
EAN: 9781607616870
No. of Pages: 480
Publish Date: 2010-6-30
Binding: Hardcover
Deliverable Countries: This product ships to United Arab Emirates, Australia, Belgium, Bahrain, Switzerland, China, Germany, Spain, Finland, France, Hong Kong, Indonesia, India, Japan, Kenya, Kuwait, Sri Lanka, Malaysia, Netherlands, New Zealand, Russia, Saudi Arabia, Singapore, Thailand, South Africa.
Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology(TM) series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.
肝脏学领域的方法学名作,MIMM系列,自行下载打包后发布与此,供肝脏药物药理、物质代谢、损伤与再生、病毒性肝病等研究方向的战友选读~
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