Directed Molecular Evolution of Proteins:

aftc

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis
By Susanne Brakmann, Kai Johnsson


Publisher:  Wiley-VCH
Number Of Pages:  368
Publication Date:  2002-05-06
ISBN-10 / ASIN:  3527304231
ISBN-13 / EAN:  9783527304233
Binding:  Hardcover

Product Description:

Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity.
The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!

http://rapidshare.com/files/43841282/brakm3527304231.rar