PCR Cloning Protocols

aftc

PCR Cloning Protocols (Methods in Molecular Biology) (Methods in Molecular Biology)
By Bing-Yuan Chen, Harry W. Janes

Publisher:  Humana Press
Number Of Pages:  460
Publication Date:  2002-04-01
ISBN-10 / ASIN:  0896039692
ISBN-13 / EAN:  9780896039698
Binding:  Hardcover

Product Description:

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

http://rapidshare.com/files/1169 ... ng_Protocols_2E.pdf