Mitochondrial DNA: Methods and Protocols

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ISBN: 0896039722
Title: Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology)
Author: by William C. Copeland (Editor)
Publisher: Humana Press
Publication Date: 2002-04
Number Of Pages: 432

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Contents:
Part I. Methods for the Analysis of mtDNA

Animal Models for Mitochondrial Disease
Douglas C. Wallace

Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
Eric A. Schon, Ali Naini, and Sara Shanske

Screening for Aging-Dependent Point Mutations in mtDNA
Yuichi Michikawa and Giuseppe Attardi

Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
Weiming Zheng, Luisa A. Marcelino, and William G. Thilly

Identification of mtDNA Mutations in Human Cancer
Shuji Nomoto, Montserrant Sanchez-Cespedes, and David Sidransky

In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis?
Steven J. Zullo

Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
Alice Wong and Gino Cortopassi

Yeast Nuclear Genes for mtDNA Maintenance
Fran鏾ise Foury

Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
Micheline K. Strand and William C. Copeland

Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
Janine H. Santos, Bhaskar S. Mandavilli, and Bennett Van Houten

Migration of mtDNA into the Nucleus
Mary K. Thorsness, Karen H. White, and Peter E. Thorsness

2D Gel Electrophoresis of mtDNA
Heather E. Lorimer

Part II. Methods for the Analysis of Proteins Involved in mtDNA Transactions

Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
Daniel F. Bogenhagen

Purification of Mitochondrial Uracil–DNA Glycosylase Using Ugi–Sepharose Affinity Chromatography
Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, and Dale W. Mosbaugh

Characterization of Specialized mtDNA Glycosylases
Simon G. Nyaga and Vilhelm A. Bohr

Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
Matthew J. Longley and William C. Copeland

Assay of mtDNA Polymerase g from Human Tissues
Robert K. Naviaux

Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase g
Carol L. Farr and Laurie S. Kaguni

Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein
Carol L. Farr and Laurie S. Kaguni

Expression, Purification and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
Kang Li and R. Sanders Williams

Recombinant Yeast mtDNA Helicases: Purification and Functional Assays
Silja Kuusk, Tiina Sedman, and Juhan Sedman

Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
Robert L. Low

Endonuclease G Isolation and Assays
Robert L. Low and Mariana Gerschenson

Targeting DNA Repair Proteins to Mitochondria
Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, and Susan P. LeDoux

In Organello Footprinting of mtDNA
Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, and William W. Hauswirth

Crosslinking of Proteins to mtDNA
Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, and Ronald A. Butow

Breeding and Genotyping of Tfam Conditional Knockout Mice
Mats Ekstrand and Nils-G鰎an Larsson