Molecular biology in cellular pathology(John Crocker Paul G. Murray)
Contents
Preface xiii
Preface to Molecular Biology in Histopathology xv
List of Contributors xvii
1 Blotting Techniques: Methodology and Applications 1
Fiona Watson and C. Simon Herrington
1.1 Introduction 1
1.2 Blotting techniques 1
1.3 References 15
2 In-situ Hybridisation in Histopathology 19
Gerald Niedobitek and Hermann Herbst
2.1 Introduction 19
2.2 Experimental conditions 20
2.3 Probes and labels 23
2.4 Controls and pitfalls 27
2.5 Double-labelling 29
2.6 Increasing the sensitivity of ISH 31
2.7 What we do in our laboratories 33
2.8 Applications of ISH: examples 35
2.9 Perspective 39
2.10 References 40
3 DNA Flow Cytometry 49
M.G. Ormerod
3.1 Introduction 49
3.2 Definitions and terms 49
3.3 Dye used for DNA analysis 50
3.4 Sample preparation for DNA analysis 52