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ISBN: 0896039722
Title: Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology)
Author: by William C. Copeland (Editor)
Publisher: Humana Press
Publication Date: 2002-04
Number Of Pages: 432
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Contents:
Part I. Methods for the Analysis of mtDNA
Animal Models for Mitochondrial Disease
Douglas C. Wallace
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
Eric A. Schon, Ali Naini, and Sara Shanske
Screening for Aging-Dependent Point Mutations in mtDNA
Yuichi Michikawa and Giuseppe Attardi
Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis
Weiming Zheng, Luisa A. Marcelino, and William G. Thilly
Identification of mtDNA Mutations in Human Cancer
Shuji Nomoto, Montserrant Sanchez-Cespedes, and David Sidransky
In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis?
Steven J. Zullo
Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™
Alice Wong and Gino Cortopassi
Yeast Nuclear Genes for mtDNA Maintenance
Fran鏾ise Foury
Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay
Micheline K. Strand and William C. Copeland
Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR
Janine H. Santos, Bhaskar S. Mandavilli, and Bennett Van Houten
Migration of mtDNA into the Nucleus
Mary K. Thorsness, Karen H. White, and Peter E. Thorsness
2D Gel Electrophoresis of mtDNA
Heather E. Lorimer
Part II. Methods for the Analysis of Proteins Involved in mtDNA Transactions
Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions
Daniel F. Bogenhagen
Purification of Mitochondrial Uracil–DNA Glycosylase Using Ugi–Sepharose Affinity Chromatography
Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, and Dale W. Mosbaugh
Characterization of Specialized mtDNA Glycosylases
Simon G. Nyaga and Vilhelm A. Bohr
Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit
Matthew J. Longley and William C. Copeland
Assay of mtDNA Polymerase g from Human Tissues
Robert K. Naviaux
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase g
Carol L. Farr and Laurie S. Kaguni
Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein
Carol L. Farr and Laurie S. Kaguni
Expression, Purification and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein
Kang Li and R. Sanders Williams
Recombinant Yeast mtDNA Helicases: Purification and Functional Assays
Silja Kuusk, Tiina Sedman, and Juhan Sedman
Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart
Robert L. Low
Endonuclease G Isolation and Assays
Robert L. Low and Mariana Gerschenson
Targeting DNA Repair Proteins to Mitochondria
Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, and Susan P. LeDoux
In Organello Footprinting of mtDNA
Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, and William W. Hauswirth
Crosslinking of Proteins to mtDNA
Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, and Ronald A. Butow
Breeding and Genotyping of Tfam Conditional Knockout Mice
Mats Ekstrand and Nils-G鰎an Larsson |
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