The bacterium cellulose is one kind by the microorganism synthesis ultramicro pure cellulose, has the cellulose with the nature in to compare, has the purity high, the crystallinity high and the degree of polymerization high, the hygroscopicity strong and the tensile strength is good, the biological compatibility may in the nature direct degeneration and so on the unique nature. Using the microorganism fermentation production bacterium cellulose, and its application with domain and so on food, medicine, papermaking and bio-medical engineering, has the widespread application prospect, is now one of biological material research hot spots. This topic is uses the Pakistan vinegar bacillus (Acetobacter pasteurianus) is the production strain synthesis bacterium cellulose, to synthesized the bacterium cellulose the method, the best synthesis condition, the different culture medium ingredient has carried on the exploration.
Through to raises the condition the comparison: Discovered under the scanning electricity mirror; Under the static raise, the bacterium cellulose along carries on by a stock connected cellulose ribbon with the major axis parallel direction layered does not have the rule to overlap with twines mutually; Under the shake culture, the cellulose piece take the three dimensional network structure as a characteristic, by adds the thick cellulose ribbon mutual connection in turn, by the three-dimensional space development, forms the check shape, the single textile fiber ribbon width is 0.05~0.1 mu m.
Through has designed one kind of special raise system in the static raise, namely has 100um in the cylinder shape vessel base shop thick to have the seepage oxygen ability the silica gel level, lets the Pakistan acetic acid fungus penetrable oxygen on the silicon cellophane and the liquid surface produces the cellulose thin film, studied has dissolved the oxygen to the bacterium cellulose synthesis influence, discovered in dissolved under the oxygen condition to be possible to cause the bacterium cellulose the output to double.
Through the research culture medium ingredient to the bacterium cellulose synthesis influence, and has discussed with emphasis take the glycerine as the only carbon source synthesis cellulose method, the best synthesis condition. The experiment discovered that, Using the glycerine took culture medium for carbon source when the bacterium growth cycle is shortest, is 2-3 day generally. The most suitable growth condition is meeting type and quantity 7. Content 31 ℃ under raises 10 day to be possible to achieve the greatest outputThe glycerine not only may do for the cellulose may the use carbon source, moreover is one kind of high efficiency, the high yield carbon source matter. Through the remaining material content determination obtains the glycerine use factor to the vaccine in is 83.2
Carries on the track test to the different C source element for the culture medium bacterium nutrient fluid PH value, discovered the different C source element has the high -> low -> high tendency for the culture medium bacterium nutrient fluid PH value change
This research not only provides the theory basis for the bacterium cellulose highly effective synthesis, moreover had found a new way for the industrial production bacterium cellulose.
Key words: Acetobacter pasteurianus .bacterium cellulose, glycerine |
发表于 2006-5-28 21:40:28
发表于 2006-5-30 16:03:17
