GST-Fusion Proteins
PHARMACIA-GST fusion protein Handbook (pdf)
poly His-Tagged Fusion Proteins
CLONTECH: BD HAT Protein Expression and Purification System User Manual
NOVAGEN: pET purification manual (pdf)
QIAGEN: Ni-NTA purification manual (pdf)
Maltose Binding Protein
NEW ENGLAND BIOLAB: pMAL protein Fusion and Purification System (pdf)
Calmodulin Binding Peptide
STRATAGENE: CBP Calmodulin-Binding Peptide Affinity Tag System
Intein Mediated Purification
NEW ENGLAND BIOLAB: INTEIN-TM System (pdf)
NEW ENGLAND BIOLAB: INTEIN-TWIN System (pdf)
Biotinylated Fusion Protein
PROMEGA PinPointTM Xa Protein Purification System (pdf)
Two Step: poly His and Strep-Tag
QIAGEN: Two-Step Affinity Purification System Handbook
For expressing, purifying, and detecting proteins carrying a 6xHis and Strep-tag II (pdf)
SUMO
LIFESENSORS SUMOpro™ Expression System T7, Amp, E.coli (pdf)
LIFESENSORS SUMOpro™ Expression System T7, Kan, E.coli (pdf)
LIFESENSORS SUMO Gene Fusion Technology - New Methods for Enhancing Protein Expression and Purification in Eukaryotes (pdf)
LIFESENSORS SUMO Gene Fusion Technology - New Methods for Enhancing Protein Expression and Purification in Prokaryotes (pdf)
LIFESENSORS SUMO Gene Fusion Technology - New Methods for Rapid Recombinant Peptide Expression and Purification (pdf)
Drosophila Expression System
INVITROGEN : Drosophila Expression System (pdf)
Eukaryote Expression System
LIFESENSORS Split SUMOpro™ Expression System CUP1, Amp, Yeast (pdf)
LIFESENSORS Split SUMOpro™ Expression System pPolh, Amp, Insect (pdf)
In-Vitro Translation System
ROCHE Rapid Translation System Products
ROCHE Rapid Translation System RTS100 (pdf)
ROCHE_Rapid Translation System RTS500 (pdf)
ROCHE_Rapid Translation System RTS500 Circular (pdf)
ROCHE_Rapid Translation System RTS9000 (pdf)
ROCHE_RTS_Amino Acid Sampler (pdf)
ROCHE_Rapid Translation System GroE Supplement (pdf)
Insect Expression System
NOVAGEN InsectDirect™ Protein Expression & Purification System (pdf)
Leishmania
JENA BIOSCIENCE LEXSY Leishmania tarentolae Expression System (pdf) (pdf-article)
Preparation of Cell Lysates / Different Disruption Methods
NOVAGENBugBaster Bacterial Protein Extraction (pdf)
NOVAGEN : Lysonase Bioprocessing Reagent (pdf)
NOVAGEN POP culture Reagent (pdf)
PIERCE B-Per Bacterial Protein Extraction Reagent (pdf)
Bacterial Protein Extraction (mini-scale) Sonication
Bacterial Protein Extraction (mini-scale) Using B-Per
PROTEIN EXPRESSION FACILITY of THE HEBREW UNIVERSITY Sonication of bacterial samples
Choice of Buffers
AMERSHAM BIOSCIENCES Recommended buffers for Anion Exchange Chromatography
AMERSHAM BIOSCIENCES Recommended buffers for Cation Exchange Chromatography
AMERSHAM BIOSCIENCES Recommended Volatile buffers systems for Ion Exchange Chromatography
AMRESCO Buffers Technical Bulletin (pdf)
CALBIOCHEM Buffers - A guide for the preparation and use of buffers in biological systems (pdf)
CALBIOCHEM Table of detergents and buffers (pdf)
PROTEIN EXPRESSION AND PURIFICATION FACILITY OF THE EUROPEAN MOLECULAR BIOLOGY LABORATORY Choice of lysis buffer and additives
PROTEIN EXPRESSION AND PURIFICATION FACILITY OF THE EUROPEAN MOLECULAR BIOLOGY LABORATORY Solubility Studies
Preparation of soluble/insoluble protein from cells
SIGMAUseful pH Ranges of Selected Biological Buffers (pdf)
DNA removal
NOVAGEN Benzonase Nuclease - Removal of Nucleic Acids (pdf)
NOVAGEN : Lysonase Bioprocessing Reagent (pdf)
Removal of Nucleic Acids - Different Protocols
VIVASCIENCE Removal of DNA from lysates of E. coli cells and French bean tissue prior to electrophoresis. (pdf)
AMERSHAM-BIOSCIENCES Handbook Protein Purification (pdf)
AMERSHAM-BIOSCIENCES Protein purification Applications (pdf)
BIO RAD Sample Preparation Handbook (pdf)
CLONTECH: Troubleshooting Guide (pdf)
Test Tube
MERCK Cleaning and Regeneraton of Fractogel EMD sorbents (pdf)
NOVAGEN Benzonase Nuclease - Removal of Nucleic Acids (pdf)
NOVAGENBuffer Compositin for Fusion Tag Affinity Purification (pdf)
Removal of Nucleic Acids - Various Protocols
Purification of Recombinant Proteins
AMERSHAM-BIOSCIENCES The Recombinant Protein Handbook (pdf)
PIERCE: Fusion Protein Fusion Protein Products (pdf)
SIGMA: DETECTION AND PURIFICATION Detection and Purification Selection Guide (pdf-I) (pdf-II)
CLONTECH: Protein Purification Products (pdf)
AMERSHAM-BIOSCIENCES GST fusion protein Handbook (pdf)
NOVAGENGST.Bind Kits(pdf)
NOVAGEN: His-Tag GST-Tag purification and Detection tools (pdf)
Small scale GST-fusion protein purification under nature conditions
poly His-Tagged Fusion Proteins
NOVAGEN: pET purification manual (pdf)
NOVAGEN: Ni-NTA Hi-Bind Resins Protocols (pdf)
NOVAGEN: His-Tag GST-Tag purification and Detection tools (pdf)
QIAGEN: Ni-NTA purification manual (pdf)
QIAGEN: NiNTA cell lysis under nature conditions (pdf)
MERCK: Metal Chelate Affinity Chromatography (pdf) (pdf-II)
CLONTECH: Talon Resin Protocol (pdf)
CLONTECH: Talon Products (pdf)
CLONTECH: BD TalonTM Metal Affinity Resins User Manual (pdf)
CLONTECH: Purification in the presence of Beta Mercaptoethanol (pdf)
CLONTECH: Protein Purification Products (pdf)
CLONTECH: BD HAT Protein Expression and Purification System User Manual (pdf)
AMERSHAM-BIOSCIENCES Hi Trap Chelating (pdf)
AMERSHAM-BIOSCIENCES Hi Trap Chelating Application Note (pdf)
AMERSHAM-BIOSCIENCES Chelating Sepharose Fast Flow Instructions (pdf)
AMERSHAM-BIOSCIENCES Ni Sepharose High Performance (pdf) (pdf-II)
ACTIVE MOTIVE: Ni-Ted Manual (pdf)
AFFILAND: Metal Purification
VIVASCIENCE: Vivapure Metal Chelate Mini Spin Columns (pdf-I) (pdf-II) (pdf-III) (pdf-IV) (pdf-V) (pdf-VI)
Small scale His-Tag fusion protein purification under nature conditions
Small scale His-Tag fusion protein purification under denaturative conditions
DYNAL Dynabeads TalonTM (pdf)
Ni-NTA: Handling and Reuse
SIGMA: His Selected Nickel Affinity Gel (pdf)
PROMEGA: MagneHisTM Protein Purification System (pdf)
HISPANAGAR Chelate Agarose Beads (pdf-I) (pdf-II) (pdf-III)
SARTORIUS Sartobind
Protein Quantitation Comparision of Different Protein Determination Methods
Absorbance 280nm According toProtein Protocols in CD Rom
Bradford
Lowry According toProtein Protocols in CD Rom
Lowry-Peterson For membrane proteins, diluted solutions or solutions with interferants
Protein Precipitation Methods to concentrate or eliminate interferences before electrophoresis or protein determination
BIO-RAD Bradford Modified BIO RAD protein Assay (pdf)
BIO-RAD Lowry Modified DC BIO-RAD (pdf) (pdf-2)
BIO-RAD Lowry Modified RC DC BIO-RAD (pdf)
GENO Non-Interfering Protein Assay GENO Technology
发表于 2005-4-21 08:27:18