12 Problems or Issues You May Encounter with Western Blotting
Poor transfer
Small protein blow-through
Visualization of proteins before immunodetection
Low specificity with high background
Noisy background
High overall background
Antibody specificity controls
Low signal/sensitivity
Difficulties with reprobing
Lengthy immunodetection process
High background autofluorescence
Unidentified artifacts on blots
发表于 2008-4-20 11:22:35