高压氧治疗脑缺血的新机制：影响白细胞功能

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Reperfusion-Induced Neutrophil CD18 Polarization: Effect of Hyperbaric Oxygen
灌注诱导的嗜中性粒细胞CD18极化：高压氧的作用
Kayvan T. Khiabani, M.D., Seth A. Bellister, B.S., Sarah S. Skaggs, Linda L. Stephenson, B.S., M.T.(A.S.C.P.), Chandra Nataraj, Ph.D., Wei Z. Wang, M.D., William A. Zamboni, M.D.

Received 1 August 2007 published online 04 March 2008.
Corrected Proof

Background
Hyperbaric oxygen (HBO) inhibits ischemia reperfusion (IR) -induced neutrophil adhesion to endothelium through an unknown mechanism. This study evaluates the effect of HBO on IR-stimulated neutrophil adhesion and polarization of expressed CD18 adhesion molecules using a novel in vitro adhesion assay and confocal microscopy.
背景
高压氧(HBO)通过一个未知的机制抑制缺血再灌注(IR)诱导的嗜中性粒细胞粘附于内皮上。本研究利用一种新的体外粘附检测和共聚焦显微镜方法评估HBO对在缺血再灌注刺激下的嗜中性粒细胞粘附和阳性CD18粘附分子极化中的作用
Materials and methods
Neutrophils from normal animals were isolated from whole blood and incubated with plasma from rat gracilis muscle flaps on coverslips pretreated with ICAM. Percent adherence to ICAM and CD18 polarization was evaluated in the following five groups: (1) Nonischemic control, n = 15; (2) 4 h ischemia (IR, n = 15); (3) 4 h ischemia with HBO treatment (100% oxygen at 2.5 atmospheres absolute (IR + HBO, n = 15)); (4) 4 h ischemia with 100% oxygen at room temperature and pressure (RTP) (IR + normobaric hyperoxia, n = 5); and (5) 4 h ischemia with 8% oxygen at 2.5 atmospheres absolute (IR + hyperbaric normoxia, n = 5). Direct HBO treatment of neutrophils was also evaluated.
材料与方法
嗜中性粒细胞从正常动物的全血中分离且用ICAM预处理过的盖玻片覆盖来自于大鼠股薄肌皮瓣的血浆孵化。ICAM的粘附率和CD18的极化率用下面五组公式来评估（1）非缺血对照，n=15；（2）缺血4小时 (IR, n = 15);（3）缺血4小时并用HBO治疗（100%氧气在2.5个绝对大气压下）(IR + HBO, n = 15);（4）缺血4小时100%氧气常温常压下(IR + 常压高氧n = 5)和（5）缺血4小时8%氧气和2.5个绝对大气压下(IR +高压常氧n = 5)。嗜中性粒细胞的直接HBO治疗也在评估中。
Results
Neutrophils exposed to IR plasma showed a significant increase in percent adherent (0.8 ± 0.1% versus 16.7 ± 2.2%, P < 0.05) and polarized cells (6.2 ± 1.7% versus 43.9 ± 12.2%, P < 0.05) compared to controls. Hyperbaric oxygen significantly reduced the adhesion and polarization to 1.6 ± 0.3 and 4.1 ± 2.5%, respectively (P = < 0.05). Normobaric hyperoxia and hyperbaric normoxia did not affect neutrophil adherence or CD18 polarization following IR. Direct HBO treatment of neutrophils did not change the percent of polarized cells in IR.
结果
暴露于IR血浆中的嗜中性粒细胞与对照组相比较粘附(0.8 ± 0.1% 对 16.7 ± 2.2%, P < 0.05)与极化(6.2 ± 1.7% 对 43.9 ± 12.2%, P < 0.05)比率有重大的增加。高压氧显著的减少粘附和极化分别至1.6 ± 0.3 and 4.1 ± 2.5%, (P = < 0.05)。常压高氧和高压常氧不影响继发于IR的嗜中性粒细胞粘附或CD18极化。嗜中性粒细胞的直接HBO治疗不能改变极化细胞在IR中的百分率。
Conclusions
Hyperbaric oxygen inhibits IR-induced neutrophil adhesion by blocking CD18 surface polarization and requires plasma exposure to HBO. Treatment with oxygen or pressure alone is not effective.
结论
高压氧通过阻止CD18表面极化和要求血浆暴露于HBO抑制IR诱导的嗜中性粒细胞粘附。单独的氧气或压力治疗是无效的。

高压氧治疗脑缺血目前有争议，本研究提供了新的证据。而且该文章对指导临床的研究具有很大价值，建议关注学习。