Lymphocytes: A Practical Approach (Practical Approach Series)

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Description:
Cellular immunology is a rapidly moving field in which recent advances have made significant

contributions to our understanding of the immune response to infection and malignancy. These in

turn, have given rise to new therapeutic opportunities in areas such as vaccines and

immunotheraphy. Many investigators have been discouraged by the complicated protocols involved

in cellular immunological studies, as illustrated by the meticulous care required for the

generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition)

contains straight-forward protocols for well- established procedures in the study of lymphocytes

including preparation and identification of lymphocytes, immortalization, cell and organ

culture, and quantification assays. It also covers the recent technological advances which have

revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA

tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without

the need for in vitro culture, and molecular methods for accurate HLA typing.

Links
http://linkgrabber.blogspot.com/2006/11/lymphocytes.html