High Resolution Chromatography

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High Resolution Chromatography: A Practical Approach (Practical Approach Series)

Publisher: Oxford University Press, USA
Number Of Pages: 336
Publication Date: 1999-06-21
Sales Rank: 5090028
ISBN / ASIN: 0199636494
EAN: 9780199636495
Binding: Hardcover
Manufacturer: Oxford University Press, USA
Studio: Oxford University Press, USA

Book Description:
The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

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