A histochemical study with aniline blue staining revealed a positive correlation between the time of external symptom development and abnormal fluorescence in phloem tissue ofVinca rosea plants grafted with mycoplasma-infectedVinca scions. A range of abnormal fluorescence was observed in phloem tissue ofVinca stems after mycoplasmas of 15 different plant origins were respectively introduced by graft-inoculation. The degree of fluorescence intensity and distribution in phloem tissue was related to the severity of disease. Results from examination of mycoplasmas from culture did not suggest that these organisms themselves contribute to the abnormal fluorescence in phloem of infectedVinca.