edited by Tom Moss
Humana Press | ISBN: 0896036251 | 2001 | 628 pages | PDF | 2.7 MB
Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein–DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA–protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA–Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA–protein interactions.
发表于 2006-11-20 22:47:05
