活动 《干细胞文献汇总与评论》
本贴宗旨：促进干细胞知识学习，提高干细胞研究水平和信息交流。
跟帖原则：
1、提交文献以SCI专业学术期刊收录的干细胞研究论著或综述等为准，期刊影响因子通常≥5。影响因子<5者需说明推荐理由。
2、采用附件方式或其他长期有效网络存贮方式提交文献，注明资源链接。
3、每次须提交1篇以上文献，附有文章全名，收录期刊名、卷(期)、页码；或者组织同类文献(见下说明)5篇以上，附有目录和适当说明；或整期文献，附有目录。
4．主要评论内容：在提交文献全文的基础上予以评论，400字以内，深入浅出、简明扼要地说明作者的研究方法、结果和科学意义，并用简短的话表明自己的观点或推荐理由。
奖励原则：1．符合以上格式和要求，单篇文献奖励1-5财富；如该文章有重大理论意义、重大技术创新，或有重大应用前景，并附有清晰评论者奖励5财富以上或1威望以上。
2．提交《细胞干细胞》、《干细胞》杂志某一整期文章；或者《nature》、《science》等国际著名期刊下某一干细胞主题或某一年文献，并附有目录者奖励2威望以上。
3．组织干细胞亚主题内同类文献如胚胎干细胞、成体干细胞、癌症干细胞、干细胞与微环境、干细胞应用、干细胞遗传基因、发育分化、干细胞技术、政策等，并且文献之间关系较密切或价值较高。组织5篇以上(包括5篇)，奖励5财富以上或1威望以上。组织10篇以上(包括10篇)奖励10财富以上或2威望以上。
4。其他符合上述格式要求的奖励1～1０财富，有自己原创思考和明显积极意义者奖励１威望以上。
处罚原则：
提交文献前需在本活动贴内查找有无重复，资源重复者；或其中有关自己原创思考和交流的内容不得抄袭，也禁止马甲重复参加，否则扣除财富或威望所得。
还有很多！
沙发。引用几篇。谢谢作者！ 糖尿病是人类一大难治疾病，其并发症对人体危害尤其严重，可引起心血管硬化、神经系统变性和感染等。患者常采用口服降糖药治疗，病情较重的患者则需注射胰岛素。如果应用细胞移植技术将能够产生激素的细胞植入胰腺原位生长，则可以避免注射胰岛素、口服药物带来的痛苦和毒副作用。据美国D’Amour等学者报道，他们模拟胰腺发育过程，成功地将胚胎干细胞诱导分化为分泌胰岛素的β细胞，产量与成人正常胰岛细胞作用相近。这为糖尿病的细胞治疗带来了美好希望。
作者在体外培养、逐步诱导分化胚胎干细胞的示意图如下：
胰岛素产量：
原文：
Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells.
Nature Biotechnology - 24(11), 1392 - 1401 (2006)
《stem cell》2007年第1期：http://www.91files.com/?ENCLY5MH3AE0GCQMS2PL
目录如下：
EDITORIAL
STEM CELLS: A Quarter Century of Progress
EMBRYONIC STEM CELLS: CHARACTERIZATION SERIES
Concise Review: Epigenetic Mechanisms Contribute to Pluripotency and Cell Lineage Determination of Embryonic Stem Cells
Ablation of Undifferentiated Human Embryonic Stem Cells: Exploiting Innate Immunity Against the Gal 1-3Gal?1-4GlcNAc-R (-Gal) Epitope
Dppa2 and Dppa4 Are Closely Linked SAP Motif Genes Restricted to Pluripotent Cells and the Germ Line
Activin A Efficiently Specifies Definitive Endoderm from Human Embryonic Stem Cells Only When Phosphatidylinositol 3-Kinase Signaling Is Suppressed
Production of Green Fluorescent Protein Transgenic Embryonic Stem Cells Using the GENSAT Bacterial Artificial Chromosome Library
Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer
The Cell Surface Glycosphingolipids SSEA-3 and SSEA-4 Are Not Essential for Human ESC Pluripotency
TISSUE-SPECIFIC STEM CELLS
Concise Review: Bone Morphogenetic Protein Pleiotropism in Neural Stem Cells and Their Derivatives—Alternative Pathways, Convergent Signals
Concise Review: Multipotent Mesenchymal Stromal Cells in Blood
Potential of Dental Mesenchymal Cells in Developing Teeth
Nitric Oxide Decreases Subventricular Zone Stem Cell Proliferation by Inhibition of Epidermal Growth Factor Receptor and Phosphoinositide-3-Kinase/Akt Pathway
Potential Treatment of Cerebral Global Ischemia with Oct-4+ Umbilical Cord Matrix Cells
In Vitro Expanded Cells Contributing to Rapid Severe Combined Immunodeficient Repopulation Activity Are CD34+38-33+90+45RA-
Essential Roles of Sphingosine 1-Phosphate/S1P1 Receptor Axis in the Migration of Neural Stem Cells Toward a Site of Spinal Cord Injury
Multilineage Differentiation and Characterization of the Human Fetal Osteoblastic 1.19 Cell Line: A Possible In Vitro Model of Human Mesenchymal Progenitors
Stem/Progenitor Cell-Specific Enhanced Green Fluorescent Protein Expression Driven by the Endogenous Mcm2 Promoter
Basal Cells of the Human Adult Airway Surface Epithelium Retain Transit-Amplifying Cell Properties
Survivin Identifies Keratinocyte Stem Cells and Is Downregulated by Anti-?1 Integrin During Anoikis
EphB/Ephrin-B Interaction Mediates Adult Stem Cell Attachment, Spreading, and Migration: Implications for Dental Tissue Repair
CANCER STEM CELLS
Molecular Profiling of CD34+ Cells in Idiopathic Myelofibrosis Identifies a Set of Disease-Associated Genes and Reveals the Clinical Significance of Wilms' Tumor Gene 1 (WT1)
EMBRYONIC STEM CELLS
Force Measurements of Human Embryonic Stem Cell-Derived Cardiomyocytes in an In Vitro Transplantation Model
STEM CELL GENETICS AND GENOMICS
Stage-Specific Conditional Mutagenesis in Mouse Embryonic Stem Cell-Derived Neural Cells and Postmitotic Neurons by Direct Delivery of Biologically Active Cre Recombinase
Higher Expression of Transcription Targets and Components of the Nuclear Factor-B Pathway Is a Distinctive Feature of Umbilical Cord Blood CD34+ Precursors
TECHNOLOGY DEVELOPMENT
N-Glycolylneuraminic Acid Xenoantigen Contamination of Human Embryonic and Mesenchymal Stem Cells Is Substantially Reversible
THE STEM CELL NICHE
Notch Signaling Induces Apoptosis in Primary Human CD34+ Hematopoietic Progenitor Cells
Identification of CXCR4 as a New Nitric Oxide-Regulated Gene in Human CD34+ Cells
TRANSLATIONAL AND CLINICAL RESEARCH
In Vivo Distribution of Human Adipose-Derived Mesenchymal Stem Cells in Novel Xenotransplantation Models
Long-Term Follow-Up of Patients with Non-Hodgkin Lymphoma Following Myeloablative Therapy and Autologous Transplantation of CD34+-Selected Peripheral Blood Progenitor Cells
Cellular Cardiomyoplasty: Improvement of Left Ventricular Function Correlates with the Release of Cardioactive Cytokines
Monocyte Chemotactic Protein-3 Is a Myocardial Mesenchymal Stem Cell Homing Factor
Placental Growth Factor-1 Potentiates Hematopoietic Progenitor Cell Mobilization Induced by Granulocyte Colony-Stimulating Factor in Mice and Nonhuman Primates 在2楼，我们说了人胚胎干细胞可以诱导分化为胰腺的胰岛素分泌细胞(β细胞)，这是非临床研究。下面这篇文章则是一篇临床应用研究，作者来自巴西圣保罗大学的Julio Voltarelli等，他们应用患者自体外周血干细胞移植方法成功治疗Ⅰ型糖尿病。
糖尿病分为二型，Ⅰ型多发于青少年，是由于患者自体免疫系统失衡，破坏胰腺β细胞，患者发病时约有90%β细胞受损。Ⅱ型糖尿病发病机制与Ⅰ型不同，是由于胰岛素抵抗或分泌缺陷，而非β细胞自身免疫破坏。二者治疗方式不同，Ⅰ型糖尿病需要注射胰岛素以维持血糖平衡。作者在应用大剂量免疫抑制剂的基础上实施造血干细胞移植，15名患者有14名摆脱了对胰岛素药品的依赖。受试患者平均随访时间有18.8个月，最长一位患者已经持续三年的时间没有注射过胰岛素了。
虽然干细胞移植取得了令人惊喜的效果，但遗憾的是我们并不知道其中的作用机制。研究显示患者免疫系统在治疗后发生改变、重建，原记忆T细胞降低，新的T细胞增加。胰岛素分泌增加来源于残留的β 细胞再生，还是胰腺干细胞和造血干细胞分化所致，尚不清楚。
即便如此，这也使我们看到了干细胞在治疗糖尿病中的重要价值。目前尚没有能够象该造血干细胞移植那样可以消除患者对胰岛素注射的依赖。但该治疗过程中的并发症以及其中的作用机制仍需要进一步去关注。
"Autologous Nonmyeloablative Hematopoietic Stem Cell Transplantation in Newly Diagnosed Type 1 Diabetes Mellitus."
Júlio C. Voltarelli, Carlos E. B. Couri, Ana B. P. L. Stracieri, et al.
JAMA. 2007, 297(4);297:1568-1576.
二十世纪末有一件轰动世界的事件，这就是多利羊的克隆成功。
1997年英国《自然》杂志公布了爱丁堡罗斯林研究所威尔莫特等人的研究成果：经过247次失败之后，他们在1996年得到了一只名为“多利”的克隆雌性小绵羊。
“多利”绵羊是如何“创造”出来的呢?威尔莫特等学者先给“苏格兰黑面羊”注射促性腺素，促使它排卵。得到卵之后，立即用极细的吸管从卵细胞中取出核。与此同时，从怀孕三个月的“芬多席特”六龄母羊的乳腺细胞中取出核，立即送入取走核的“苏格兰黑面羊”的卵细胞中。手术完成之后，用相同频率的电脉冲刺激换核卵，让“苏格兰黑面羊”的卵细胞质与“芬多席特”母羊乳腺细胞的核相互协调，使这个“组装”细胞在试管里经历受精卵那样的分裂、发育而形成胚胎的过程。然后，将胚胎巧妙地植入另一只母羊的子宫里。到去年7月，这只“护理”体外形成胚胎的母羊终于产下了小绵羊“多利”。“多利”不是由母羊的卵细胞和公羊的精细胞受精的产物，而是“换核卵”一步一步发展的结果，因此是“克隆羊”。
克隆技术会将给人类带来极大的好处。如改善物种、治疗疾病、挽救珍稀动物等。
多利和其母亲：
多利羊克隆的步骤：
Viable offspring derived from fetal and adult mammalian cells
I. Wilmut, A. E. Schnieke*, J. McWhir, A. J. Kind* & K. H. S. Campbell
Nature 385, 810 - 813 (27 February 1997); doi:10.1038/385810a0
看来这个版块也许有点专业了。只要英文功底好，该话题太好挣分了!
期待大家踊跃参与 非常支持楼住的这项活动，本人是从事干细胞研究的新手，很高兴能在这里和各位共同学习
经典文献推荐 引用第7楼zhaichao_1于2008-06-03 11:25发表的 :
非常支持楼住的这项活动，本人是从事干细胞研究的新手，很高兴能在这里和各位共同学习
经典文献推荐
期待评论，做到学习、挣钱两不误才是正道。 引用第8楼orientsir于2008-06-03 14:50发表的 :
期待评论，做到学习、挣钱两不误才是正道。
谢谢楼主,提醒
只是新人,也刚学习
谈不上评论,更多是求教
Nature Cell Biology 10, 731 - 739 (2008)
Nature Cell Biology 10, 731 - 739 (2008)Published online: 4 May 2008 | doi:10.1038/ncb1736Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells
Jiancong Liang1,6, Ma Wan1,6, Yi Zhang1, Peili Gu2,4, Huawei Xin1, Sung Yun Jung1, Jun Qin1,2, Jiemin Wong2,5, Austin J. Cooney2,3, Dan Liu1 & Zhou Songyang1,3
Top of pageNanog and Oct4 are essential transcription factors that regulate self-renewal and pluripotency of ES cells. However, the mechanisms by which Nanog and Oct4 modulate ES cell fate remain unknown. Through characterization of endogenous Nanog and Oct4 protein complexes in mouse ES cells, we found that these transcription factors interact with each other and associate with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes. In addition, Nanog, Oct4 and repressor proteins co-occupy Nanog-target genes in mouse ES cells, suggesting that Nanog and Oct4 together may communicate with distinct repression complexes to control gene transcription. To our surprise, of the various core components in the NuRD complex with which Nanog and Oct4 interact, Mta1 was preferred, whereas Mbd3 and Rbbp7 were either absent or present at sub-stoichiometric levels. We named this unique Hdac1/2- and Mta1/2-containing complex NODE (for Nanog and Oct4 associated deacetylase). Interestingly, NODE contained histone deacetylase (HDAC) activity that seemed to be comparable to NuRD, and retained its association with Nanog and Oct4 in Mbd3- /- ES cells. In contrast to Mbd3 loss-of-function, knockdown of NODE subunits led to increased expression of developmentally regulated genes and ES-cell differentiation. Our data collectively suggest that Nanog and Oct4 associate with unique repressor complexes on their target genes to control ES cell fate.
Top of page
--------------------------------------------------------------------------------
Volume 26, Number 5, 2008
要全文可短信告诉我。Other Issues:
Contents: Volume 26, Number 5, 2008EDITORIAL
EMBRYONIC STEM CELLS
STEM CELL GENOMICS AND PROTEOMICS
THE STEM CELL NICHE
TISSUE-SPECIFIC STEM CELLS
TRANSLATIONAL AND CLINICAL RESEARCH
LETTER TO THE EDITOR
Find articles in this issue containing these words:
--------------------------------------------------------------------------------
To see an article, click its link. To review many abstracts, check the boxes to the left of the titles you want, and click the 'Get All Checked Abstract(s)' button. To see one abstract at a time, click its link.
--------------------------------------------------------------------------------
EDITORIAL
STEM CELLS Appoints Four Associate Editors
Donald G. Phinney, Miodrag Stojkovic
Stem Cells May 1 2008: 1095 - 1096 . OPEN ACCESS ARTICLE
--------------------------------------------------------------------------------
EMBRYONIC STEM CELLS
Hedgehog Serves as a Mitogen and Survival Factor During Embryonic Stem Cell Neurogenesis
Chunyu Cai, Jeffrey Thorne, Laura Grabel
Stem Cells May 1 2008: 1097 - 1108 . OPEN ACCESS ARTICLE
Alkaline Phosphatase-Positive Colony Formation Is a Sensitive, Specific, and Quantitative Indicator of Undifferentiated Human Embryonic Stem Cells
Michael D. O'Connor, Melanie D. Kardel, Ioulia Iosfina, David Youssef, Min Lu, Michael M. Li, Suzanne Vercauteren, Andras Nagy, Connie J. Eaves
Stem Cells May 1 2008: 1109 - 1116 . OPEN ACCESS ARTICLE
Efficient Differentiation of Functional Hepatocytes from Human Embryonic Stem Cells
Sadhana Agarwal, Katherine L. Holton, Robert Lanza
Stem Cells May 1 2008: 1117 - 1127 . OPEN ACCESS ARTICLE
A Comparison of Protocols Used to Generate Insulin-Producing Cell Clusters from Mouse Embryonic Stem Cells
Ashleigh S. Boyd, Douglas C. Wu, Yasuyuki Higashi, Kathryn J. Wood
Stem Cells May 1 2008: 1128 - 1137 .
Leukotriene Synthesis Is Required for Hedgehog-Dependent Neurite Projection in Neuralized Embryoid Bodies but Not for Motor Neuron Differentiation
Maarten F. Bijlsma, Maikel P. Peppelenbosch, C. Arnold Spek, Henk Roelink
Stem Cells May 1 2008: 1138 - 1145 .
Lysophosphatidic Acid Inhibits Neuronal Differentiation of Neural Stem/Progenitor Cells Derived from Human Embryonic Stem Cells
Mirella Dottori, Jessie Leung, Ann M. Turnley, Alice Pébay
Stem Cells May 1 2008: 1146 - 1154 .
BAF250B-Associated SWI/SNF Chromatin-Remodeling Complex Is Required to Maintain Undifferentiated Mouse Embryonic Stem Cells
Zhijiang Yan, Zhong Wang, Lioudmila Sharova, Alexei A. Sharov, Chen Ling, Yulan Piao, Kazuhiro Aiba, Ryo Matoba, Weidong Wang, Minoru S. H. Ko
Stem Cells May 1 2008: 1155 - 1165 .
Instability of Retroviral DNA Methylation in Embryonic Stem Cells
Shigeru Minoguchi, Hideo Iba
Stem Cells May 1 2008: 1166 - 1173 .
--------------------------------------------------------------------------------
STEM CELL GENOMICS AND PROTEOMICS
Epigenetic Marking Prepares the Human HOXA Cluster for Activation During Differentiation of Pluripotent Cells
Stuart P. Atkinson, Christoph M. Koch, Gayle K. Clelland, Sarah Willcox, Joanna C. Fowler, Rebecca Stewart, Majlinda Lako, Ian Dunham, Lyle Armstrong
Stem Cells May 1 2008: 1174 - 1185 .
Functional Network Reconstruction Reveals Somatic Stemness Genetic Maps and Dedifferentiation-Like Transcriptome Reprogramming Induced by GATA2
Tse-Shun Huang, Jui-Yu Hsieh, Yu-Hsuan Wu, Chih-Hung Jen, Yang-Hwei Tsuang, Shih-Hwa Chiou, Jukka Partanen, Heidi Anderson, Taina Jaatinen, Yau-Hua Yu, Hsei-Wei Wang
Stem Cells May 1 2008: 1186 - 1201 .
--------------------------------------------------------------------------------
THE STEM CELL NICHE
Activation of Wnt Signaling in Hematopoietic Regeneration
Kendra L. Congdon, Carlijn Voermans, Emily C. Ferguson, Leah N. DiMascio, Mweia Uqoezwa, Chen Zhao, Tannishtha Reya
Stem Cells May 1 2008: 1202 - 1210 . OPEN ACCESS ARTICLE
Carboxypeptidase M Expressed by Human Bone Marrow Cells Cleaves the C-Terminal Lysine of Stromal Cell-Derived Factor-1: Another Player in Hematopoietic Stem/Progenitor Cell Mobilization?
Leah Marquez-Curtis, Ali Jalili, Kathleen Deiteren, Neeta Shirvaikar, Anne-Marie Lambeir, Anna Janowska-Wieczorek
Stem Cells May 1 2008: 1211 - 1220 .
Induction of Neurogenesis in Nonconventional Neurogenic Regions of the Adult Central Nervous System by Niche Astrocyte-Produced Signals
Jianwei Jiao, Dong Feng Chen
Stem Cells May 1 2008: 1221 - 1230 .
Mobilization of Hematopoietic Progenitor Cells by Yeast-Derived β-Glucan Requires Activation of Matrix Metalloproteinase-9
Daniel E. Cramer, Stephanie Wagner, Bing Li, Jingjing Liu, Richard Hansen, Ryan Reca, Wan Wu, Ewa Zuba Surma, Damian A. Laber, Mariusz Z. Ratajczak, Jun Yan
Stem Cells May 1 2008: 1231 - 1240 .
--------------------------------------------------------------------------------
TISSUE-SPECIFIC STEM CELLS
Characterization of Bipotential Epidermal Progenitors Derived from Human Sebaceous Gland: Contrasting Roles of c-Myc and β-Catenin
Cristina Lo Celso, Melanie A. Berta, Kristin M. Braun, Michaela Frye, Stephen Lyle, Christos C. Zouboulis, Fiona M. Watt
Stem Cells May 1 2008: 1241 - 1252 . OPEN ACCESS ARTICLE
Reciprocal Intraepithelial Interactions Between TP63 and Hedgehog Signaling Regulate Quiescence and Activation of Progenitor Elaboration by Mammary Stem Cells
Na Li, Samer Singh, Pratima Cherukuri, Hua Li, Ziqiang Yuan, Leif W. Ellisen, Baolin Wang, David Robbins, James DiRenzo
Stem Cells May 1 2008: 1253 - 1264 .
Hes1 Regulates Corneal Development and the Function of Corneal Epithelial Stem/Progenitor Cells
Takahiro Nakamura, Toshiyuki Ohtsuka, Eiichi Sekiyama, Leanne J. Cooper, Hiroshi Kokubu, Nigel J. Fullwood, Yann Barrandon, Ryoichiro Kageyama, Shigeru Kinoshita
Stem Cells May 1 2008: 1265 - 1274 .
Immunogenicity of Human Mesenchymal Stem Cells in HLA-Class I-Restricted T-Cell Responses Against Viral or Tumor-Associated Antigens
Fabio Morandi, Lizzia Raffaghello, Giovanna Bianchi, Francesca Meloni, Annalisa Salis, Enrico Millo, Soldano Ferrone, Vincenzo Barnaba, Vito Pistoia
Stem Cells May 1 2008: 1275 - 1287 .
Glycogen Synthase Kinase-3β Inhibition Preserves Hematopoietic Stem Cell Activity and Inhibits Leukemic Cell Growth
Tiffany Holmes, Tracey A. O'Brien, Robert Knight, Robert Lindeman, Sylvie Shen, Emma Song, Geoff Symonds, Alla Dolnikov
Stem Cells May 1 2008: 1288 - 1297 .
Horizontal Basal Cells Are Multipotent Progenitors in Normal and Injured Adult Olfactory Epithelium
Naomi Iwai, Zhijian Zhou, Dennis R. Roop, Richard R. Behringer
Stem Cells May 1 2008: 1298 - 1306 .
Bone Marrow Multipotent Mesenchymal Stromal Cells Do Not Reduce Fibrosis or Improve Function in a Rat Model of Severe Chronic Liver Injury
Adriana B. Carvalho, Luiz Fernando Quintanilha, Juliana V. Dias, Bruno D. Paredes, Elida G. Mannheimer, Felipe G. Carvalho, Karina D. Asensi, Bianca Gutfilen, Lea Mirian B. Fonseca, Celia Maria C. Resende, Guilherme F. M. Rezende, Christina M. Takiya, Antonio Carlos Campos de Carvalho, Regina C. S. Goldenberg
Stem Cells May 1 2008: 1307 - 1314 .
Evolution of the c-kit-Positive Cell Response to Pathological Challenge in the Myocardium
Jenna Fransioli, Brandi Bailey, Natalie A. Gude, Christopher T. Cottage, John A. Muraski, Gregory Emmanuel, Weitao Wu, Roberto Alvarez, Marta Rubio, Sergio Ottolenghi, Erik Schaefer, Mark A. Sussman
Stem Cells May 1 2008: 1315 - 1324 .
Metabolic Flexibility Permits Mesenchymal Stem Cell Survival in an Ischemic Environment
Louise A. Mylotte, Angela M. Duffy, Mary Murphy, Timothy O'Brien, Afshin Samali, Frank Barry, Eva Szegezdi
Stem Cells May 1 2008: 1325 - 1336 .
Conditional Stabilization of β-Catenin Expands the Pool of Lung Stem Cells
Susan D. Reynolds, Anna C. Zemke, Adam Giangreco, Brian L. Brockway, Roxana M. Teisanu, Jeffrey A. Drake, Thomas Mariani, Peter Y.P. Di, Mark M. Taketo, Barry R. Stripp
Stem Cells May 1 2008: 1337 - 1346 .
Embryonic Porcine Liver as a Source for Transplantation: Advantage of Intact Liver Implants over Isolated Hepatoblasts in Overcoming Homeostatic Inhibition by the Quiescent Host Liver
Helena Katchman, Orna Tal, Smadar Eventov-Friedman, Elias Shezen, Anna Aronovich, Dalit Tchorsh, Sivan Cohen, Alexander Shtabsky, Gil Hecht, Benjamin Dekel, Enrique Freud, Yair Reisner
Stem Cells May 1 2008: 1347 - 1355 .
--------------------------------------------------------------------------------
TRANSLATIONAL AND CLINICAL RESEARCH
Transplantation of Embryonic Stem Cells Improves Nerve Repair and Functional Recovery After Severe Sciatic Nerve Axotomy in Rats
Lin Cui, Jun Jiang, Ling Wei, Xin Zhou, Jamie L. Fraser, B. Joy Snider, Shan Ping Yu
Stem Cells May 1 2008: 1356 - 1365 .
In Vitro Model of Bromodeoxyuridine or Iron Oxide Nanoparticle Uptake by Activated Macrophages from Labeled Stem Cells: Implications for Cellular Therapy
Edyta Pawelczyk, Ali S. Arbab, Aneeka Chaudhry, Arun Balakumaran, Pamela G. Robey, Joseph A. Frank
Stem Cells May 1 2008: 1366 - 1375 .
Statin and Stromal Cell-Derived Factor-1 Additively Promote Angiogenesis by Enhancement of Progenitor Cells Incorporation into New Vessels
Hongwei Shao, Yaohong Tan, Darwin Eton, Zhe Yang, M. Georgina Uberti, Sen Li, Andrew Schulick, Hong Yu
Stem Cells May 1 2008: 1376 - 1384 .
--------------------------------------------------------------------------------
LETTER TO THE EDITOR
Bone Marrow-Derived Stem Cells Do Not Reconstitute Spermatogenesis In Vivo
Bruno Lassalle, Marc Andrée Mouthon, Lydia Riou, Vilma Barroca, Mathieu Coureuil, Fran鏾is Boussin, Jacques Testart, Isabelle Allemand, Pierre Fouchet
Stem Cells May 1 2008: 1385 - 1386 .
Re:Nature Cell Biology 10, 731 - 739 (2008)
However, the mechanisms by which Nanog and Oct4 modulate ES cell fate remain unknown........
what a surprise answer
ES cells have an innate programme for self-replication that does not require extrinsic instruction.
full text A core Klf circuitry regulates self-renewal of embryonic stem cells
Jianming Jiang1,2, Yun-Shen Chan1,2,6, Yuin-Han Loh1,2,6, Jun Cai3,6, Guo-Qing Tong4,5, Ching-Aeng Lim1,2, Paul Robson2,4, Sheng Zhong3 & Huck-Hui Ng1,2
--------------------------------------------------------------------------------
AbstractEmbryonic stem (ES) cells are unique in their ability to self-renew indefinitely and maintain pluripotency. These properties require transcription factors that specify the gene expression programme of ES cells. It has been possible to reverse the highly differentiated state of somatic cells back to a pluripotent state with a combination of four transcription factors: Klf4 is one of the reprogramming factors required, in conjunction with Oct4, Sox2 and c-Myc. Maintenance of self-renewal and pluripotency of ES cells requires Oct4, Sox2 and c-Myc, but Klf4 is dispensable. Here, we show that Krüppel-like factors are required for the self-renewal of ES cells. Simultaneous depletion of Klf2, Klf4 and Klf5 lead to ES cell differentiation. Chromatin immunoprecipitation coupled to microarray assay reveals that these Klf proteins share many common targets of Nanog, suggesting a close functional relationship between these factors. Expression analysis after triple RNA interference (RNAi) of the Klfs shows that they regulate key pluripotency genes, such as Nanog. Taken together, our study provides new insight into how the core Klf circuitry integrates into the Nanog transcriptional network to specify gene expression that is unique to ES cells.
胚胎干细胞的独特之处是在于他们的无限自我更新能力和全能性。这些特点要求转录因子去特化ES细胞的基因表达程序。高度分化的体细胞已经能够通过四种转录因子逆转回全能干细胞状态， klf4，oct4,Sox2，和c-Myc。自我更新的维持和ES细胞的多能性需要oct4,Sox2，和c-Myc，而Klf4并非是必需因子。这里，我们报道胚胎干细胞的自我更新需要klf因子。同时消除klf2, klf4,和klf5的基因表达回引起干细胞分化。CHIP结合芯片技术显示这些klf因子与Nanog具有相同的目标基因，提示了这些因子之间功能上的紧密联系。用三重RNAi的表达分析发现，这些因子调节包括Nanog基因在内的重要的多能性基因。综合这些结果，我们的研究提供一种新的视角，即核心klf环路整合进Nanog转录因子网络并指导胚胎干细胞专一的基因表达。 紧接楼上，把原文补上来。 Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells
Julie K. Stock1,6, Sara Giadrossi2,6, Miguel Casanova3, Emily Brookes1, Miguel Vidal4, Haruhiko Koseki5, Neil Brockdorff3, Amanda G. Fisher2 & Ana Pombo1
Changes in phosphorylation of the carboxy-terminal domain (CTD) of RNA polymerase II (RNAP) are associated with transcription initiation, elongation and termination1, 2, 3. Sites of active transcription are generally characterized by hyperphosphorylated RNAP, particularly at Ser 2 residues, whereas inactive or poised genes may lack RNAP or may bind Ser 5-phosphorylated RNAP at promoter proximal regions. Recent studies have demonstrated that silent developmental regulator genes have an unusual histone modification profile in ES cells, being simultaneously marked with Polycomb repressor-mediated histone H3K27 methylation, and marks normally associated with gene activity4, 5. Contrary to the prevailing view, we show here that this important subset of developmental regulator genes, termed bivalent genes, assemble RNAP complexes phosphorylated on Ser 5 and are transcribed at low levels. We provide evidence that this poised RNAP configuration is enforced by Polycomb Repressor Complex (PRC)-mediated ubiquitination of H2A, as conditional deletion of Ring1A and Ring1B leads to the sequential loss of ubiquitination of H2A, release of poised RNAP, and subsequent gene de-repression. These observations provide an insight into the molecular mechanisms that allow ES cells to self-renew and yet retain the ability to generate multiple lineage outcomes.
RNA聚合酶（RNAP）的羧基端（CTD）的磷酸化改变转录起始、延长、和终止有关。活跃的转录起始位点通常是高磷酸化的RNAP， 特别是在Ser2残基；而不活跃的或静息的基因可能缺乏RNAP或可能近端启动子结合有Ser 5-磷酸化的RNAP。近来的研究证明，沉默的发育调节基因在胚胎干细胞中具有特俗的组蛋白修饰——同时具有polycomb抑制子介导的H3K27甲基化和正常基因活性相关的标记。同流行的观点不同，我们的结果显示重要的发育调节基因（双活性基因）聚集着Ser5磷酸化的RNAP复合物，并且在低水平转录。我们证实这种自稳态的RNAP构型是由于Polycomb repressor complex(PRC)介导的H2A的泛素化，因为条件性敲出Ring1A和Ring1B导致了基因的去沉默。这些结果是人们能够洞察到胚胎肝细胞能够自我更新而又能够保持分化出多种细胞系的能力。
页:
[1]