Real-time PCR(PDF,4.31M)
The book is organized in two parts. The first part begins with a general introduction which is followed by chapters on the basics of data analysis, quantification, normalization and principles of primer and probe design, all contributed by leaders in the field. This part is concluded by chapters on more applied aspects of real-time PCR including an overview of high-resolution melting analysis by the most experienced users of this method. The second part of the book covers specific applications including the less recognized uses of real-time PCR: methylation detection, mitochondrial DNA analysis and immuno-PCR. The following chapters summarize many uses of real-time PCR in the clinic with examples. Clinical microbiology and virology, solid organ and bone marrow transplantation, and prenatal genetic diagnosis are among the topics covered. These chapters have been written with the laboratory and practical uses in mind and the contributors share their most valuable experiences. A comprehensive glossary and index supplement the 17 chapters and aim to make the book more accessible.
Real-time PCR is, like any other modern method in molecular genetics, expanding, with potential applications even in proteomics. This book is expected to provide the basic principles of applied real-time PCR and provide a firm grounding for those who wish to develop further applications. The selection of the chapters reflects the acknowledgment of inevitable future developments. We look forward to covering those in future editions of the book. Realtime PCR is surely here to stay and hopefully this book will help current users of this technique and also help develop further applications for it.
推荐做定量PCR的读一读，从原理到细节都讲得很清楚，应该是你一直寻找的参考书
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