aqi96 发表于 2008-4-20 11:22:35

12 Proven Strategies to Optimize Your Western Blots

12 Problems or Issues You May Encounter with Western Blotting

Poor transfer
Small protein blow-through
Visualization of proteins before immunodetection
Low specificity with high background
Noisy background
High overall background
Antibody specificity controls
Low signal/sensitivity
Difficulties with reprobing
Lengthy immunodetection process
High background autofluorescence
Unidentified artifacts on blots
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